In Vivo Translation of the Triple Gene Block of Potato Virus X Requires Two Subgenomic mRNAs

Author:

Verchot Jeanmarie1,Angell Susan M.1,Baulcombe David C.1

Affiliation:

1. Sainsbury Laboratory, Norwich Research Park, Colney, United Kingdom NR4 7UH

Abstract

ABSTRACT The 25-kilodalton (25K), 12K, and 8K movement proteins of potato virus X are derived from overlapping open reading frames (ORFs). Using an in vivo complementation assay, we have shown that the 25K protein is expressed from a functionally monocistronic mRNA, whereas the 12K and 8K proteins are from a bicistronic mRNA. Translation of the 8K ORF is by leaky ribosome scanning through the 12K ORF.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

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