Localization of a Baculovirus-Induced Chitinase in the Insect Cell Endoplasmic Reticulum-Reference-Cited by-同舟云学术

Localization of a Baculovirus-Induced Chitinase in the Insect Cell Endoplasmic Reticulum

Published:1998-12 Issue:12 Volume:72 Page:10207-10212
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Thomas Carole J.¹²,Brown Helen L.²,Hawes Chris R.²,Lee Bum Yong³,Min Mi-Kyung³,King Linda A.²,Possee Robert D.¹

Affiliation:

1. NERC Institute of Virology and Environmental Microbiology, Oxford OX1 3SR,1 and

2. School of Biological and Molecular Sciences, Oxford Brookes University, Oxford OX3 0BP,2 United Kingdom, and

3. MOGAM Biotechnology Research Institute, Yongin-Kun, Kyonggi-Do 449-910, Korea3

Abstract

ABSTRACT Confocal immunofluorescence microscopy was used to demonstrate that the Autographa californica nucleopolyhedrovirus (Ac M NPV) chitinase was localized within the endoplasmic reticulum (ER) of virus-infected insect cells. This was consistent with removal of the signal peptide from the chitinase and an ER localization motif (KDEL) at the carboxyl end of the protein. Chitinase release from cells, a prerequisite for liquefaction of virus-infected insect larvae, appears to be aided by synthesis of the p10 protein. Deletion of p10 from the Ac M NPV genome delayed the appearance of chitinase activity in the medium of virus-infected cells by 24 h and also delayed liquefaction of virus-infected Trichoplusia ni larvae by the same period.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.72.12.10207-10212.1998

Reference23 articles.

1. The sequence of the Orgyia pseudotsugata multinucleocapsid nuclear polyhedrosis virus genome;Ahrens C. H.;Virology,1997

2. The complete DNA sequence of Autographa californica nuclear polyhedrosis virus;Ayres M. D.;Virology,1994

3. Infection mechanisms of Botrytis species: pre-penetration and pre-infection processes of dry and wet conidia;Cole L.;Mycol. Res.,1996

4. Recycling of proteins from the Golgi compartment to the ER in yeast;Dean N.;J. Cell Biol.,1990

5. Plant and mammalian sorting signals for protein retention in the endoplasmic reticulum contain a conserved epitope;Denecke J.;EMBO J.,1992

Cited by 63 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. StaufenC facilitates utilization of the ERAD pathway to transport dsRNA through the endoplasmic reticulum to the cytosol;Proceedings of the National Academy of Sciences;2024-06-17

2. A distinct lineage of giant viruses brings a rhodopsin photosystem to unicellular marine predators;Proceedings of the National Academy of Sciences;2019-09-23

3. The deletion of the AcMNPV ac124 gene resulted in a decrease in chitinase transcription;Virus Research;2019-04

4. Baculovirus-driven protein expression in insect cells: A benchmarking study;Journal of Structural Biology;2018-08

5. The interaction between baculoviruses and their insect hosts;Developmental & Comparative Immunology;2018-06