Affiliation:
1. Department of Microbiology, University of Georgia, Athens, Georgia 30602
Abstract
ABSTRACT
The genome of
Myxococcus xanthus
encodes lipolytic enzymes in three different families: patatin lipases, α/β hydrolases, and GDSL lipases. One member of each family was characterized. The protein encoded by MXAN_3852 contains motifs characteristic of patatins. MXAN_5522 encodes a protein with the G-X-S-X-G motif characteristic of the lipase subfamily of α/β hydrolases. MXAN_4569 encodes a member of the GDSL family of lipolytic enzymes. Strains with deletions of MXAN_5522 and MXAN_4569 undergo faster development and earlier myxospore formation than the wild-type strain. The MXAN_5522 mutation results in spore yields substantially higher than those seen for wild-type cells. Gene expression analysis using translational
lacZ
fusions indicates that while all three genes are expressed during development, only MXAN_5522 and MXAN_4569 are expressed during vegetative growth. The proteins encoded by these genes were overexpressed using a T7 RNA polymerase transcription (pET102/D-TOPO) system in
Escherichia coli
BL21 Star (DE3) cells. The substrate specificities of the purified enzymes were investigated using
p
-nitrophenyl esters with chain lengths from C
2
to C
16
. These enzymes preferentially hydrolyzed esters of short-chain fatty acids, yielding the highest activity with
p
-nitrophenyl acetate.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
21 articles.
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