Development of Molecular Methods for Rapid Differentiation of Mycoplasma gallisepticum Vaccine Strains from Field Isolates-Reference-Cited by-同舟云学术

Development of Molecular Methods for Rapid Differentiation of Mycoplasma gallisepticum Vaccine Strains from Field Isolates

Published:2019-06 Issue:6 Volume:57 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Sulyok Kinga M.¹,Kreizinger Zsuzsa¹,Bekő Katinka¹,Forró Barbara¹,Marton Szilvia¹,Bányai Krisztián¹,Catania Salvatore²,Ellis Christine³,Bradbury Janet³,Olaogun Olusola M.⁴,Kovács Áron B.¹,Cserép Tibor⁵,Gyuranecz Miklós¹⁶

Affiliation:

1. Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, Hungary

2. Instituto Zooprofilattico Sperimentale delle Venezie, Verona, Italy

3. Institute of Veterinary Science, University of Liverpool, Leahurst Campus, Neston, United Kingdom

4. Faculty of Veterinary and Agricultural Sciences, University of Melbourne, Victoria, Australia

5. MSD Animal Health, Walton Manor, Milton Keynes, Buckinghamshire, United Kingdom

6. Department of Microbiology and Infectious Diseases, University of Veterinary Medicine, Budapest, Hungary

Abstract

Mycoplasma gallisepticum is among the most economically significant mycoplasmas causing production losses in poultry. Seven melt-curve and agarose gel-based mismatch amplification mutation assays (MAMAs) and one PCR are provided in the present study to distinguish the M. gallisepticum vaccine strains and field isolates based on mutations in the crmA , gapA , lpd, plpA , potC , glpK , and hlp2 genes.

Funder

National Research, Development and Innovation Office

Ministry of Human Capacities

Magyar Tudományos Akadémia

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01084-18

Reference38 articles.

1. Control of avian mycoplasmoses by vaccination