Affiliation:
1. Scripps Institution of Oceanography, University of California in San Diego, La Jolla, California 92093-0202
Abstract
ABSTRACT
Pseudomonas putida
MnB1 is an isolate from an Mn oxide-encrusted pipeline that can oxidize Mn(II) to Mn oxides. We used transposon mutagenesis to construct mutants of strain MnB1 that are unable to oxidize manganese, and we characterized some of these mutants. The mutants were divided into three groups: mutants defective in the biogenesis of
c
-type cytochromes, mutants defective in genes that encode key enzymes of the tricarboxylic acid cycle, and mutants defective in the biosynthesis of tryptophan. The mutants in the first two groups were cytochrome
c
oxidase negative and did not contain
c
-type cytochromes. Mn(II) oxidation capability could be recovered in a
c
-type cytochrome biogenesis-defective mutant by complementation of the mutation.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
83 articles.
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