Quantitative Measure of Small-Subunit rRNA Gene Sequences of the Kingdom Korarchaeota

Author:

Brunk Clifford F.12,Eis Nicole1

Affiliation:

1. Lehrstuhl für Mikrobiologie und Archaeenzentrum der Universität Regensburg, Regensburg, Germany,1 and

2. Biology Department and Molecular Biology Institute, University of California, Los Angeles, California2

Abstract

ABSTRACT Comparative PCR amplification of small-subunit (SSU) rRNA gene (rDNA) sequences indicates substantial preferential PCR amplification of pJP27 sequences with korarchaeote-specific PCR primers. The coamplification of a modified SSU rDNA sequence can be used as an internal standard to determine the amount of a specific SSU rDNA sequence.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference18 articles.

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2. Remarkable archaeal diversity detected in a Yellowstone National Park hot spring environment.;Barns;Proc. Natl. Acad. Sci. USA,1994

3. Perspectives on archaeal diversity, theromophily and monophyly from environmental rRNA sequences.;Barns;Proc. Natl. Acad. Sci. USA,1996

4. Absolute messenger RNA quantification using the polymerase chain reaction (PCR)—a novel approach by a PCR aided transcript titration assay (PATTY).;Becker-André;Nucleic Acids Res.,1989

5. Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli.;Brosius;J. Mol. Biol.,1981

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