Affiliation:
1. Departments of Microbiology1 and
2. Chemical and Bioresource Engineering,2 Center for Environmental Toxicology and Technology, Colorado State University, Fort Collins, Colorado 80523
Abstract
ABSTRACT
Species-specific sequences were identified within the V4 variable region of 16S rRNA of two bacterial species capable of aromatic hydrocarbon metabolism, Pseudomonas putida F1 andBurkholderia sp. strain JS150, and a third, Bacillus subtilis ATCC 7003, that can function as a secondary degrader. Fluorescent in situ hybridization (FISH) with species-specific oligonucleotides was used for direct counting of these species throughout a phenol biodegradation experiment in batch culture. Traditional differential plate counting methods could not be used due to the similar metabolism and interactions of the primary degraders and difficulties in selecting secondary degraders in mixed culture. In contrast, the FISH method provided reliable quantitative results without interference from those factors.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference30 articles.
1. The Oligonucleotide Probe Database.;Alm;Appl. Environ. Microbiol.,1996
2. Phylogenetic identification and in situ detection of individual microbial cells without cultivation.;Amann;Microbiol. Rev.,1995
3. Molecular approaches for environmental monitoring of microorganisms.;Atlas;BioTechniques,1992
4. GenBank.;Benson;Nucleic Acids Res.,1993
5. RAPD-PCR and SSCP analysis for insect population genetic studies;Black,1997
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