Ink and Vinegar, a Simple Staining Technique for Arbuscular-Mycorrhizal Fungi-Reference-Cited by-同舟云学术

Ink and Vinegar, a Simple Staining Technique for Arbuscular-Mycorrhizal Fungi

Published:1998-12-01 Issue:12 Volume:64 Page:5004-5007
ISSN:1098-5336
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl. Environ. Microbiol.

Author:

Vierheilig Horst¹²,Coughlan Andrew P.¹,Wyss Urs²,Piché Yves¹

Affiliation:

1. Centre de Recherche en Biologie Forestiére, Université Laval, Québec G1K 7P4, Canada,1 and

2. Institut für Phytopathologie, Christian-Albrecht-Universität Kiel, D-24118 Kiel, Germany2

Abstract

ABSTRACT We developed a reliable, inexpensive, and simple method for staining arbuscular-mycorrhizal fungal colonizations in root tissues. Apart from applications in research, this nontoxic, high-quality staining method also could be of great utility in teaching exercises. After adequate clearing with KOH, an ink-vinegar solution successfully stained all fungal structures, rendering them clearly visible.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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