Affiliation:
1. Departments of Biochemistry and Genetics, University of Wisconsin, Madison, Wisconsin 53706
Abstract
From a stock of ϰ phage grown on
Salmonella
, a host-range mutant which attacks
Escherichia coli
was isolated. As in the case of
Salmonella
, only motile strains of
E. coli
are sensitive to ϰ. The phage shows an eclipse period of 35 min and a minimal latent period of 52 min. The adsorption rate constant is 3 × 10
−9
ml/min. Adsorption shows a marked dependence on temperature. Bacteriophage ϰ was purified by differential centrifugation and CsCl density gradient centrifugation. It contains deoxyribonucleic acid (DNA) which is double-stranded. The DNA has a molecular weight of 42 million and a guanine plus cytosine content of 57%. Of 68 molecules of DNA inspected, 7 were circular. The phage particle weight is about 90 million.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
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