Use of the tna Operon as a New Molecular Target for Escherichia coli Detection

Author:

Bernasconi Camilla1,Volponi Giorgio2,Picozzi Claudia2,Foschino Roberto2

Affiliation:

1. European Commission, Joint Research Centre, Institute for Health and Consumer Protection, Ispra (VA), Italy

2. DiSTAM, Università degli Studi di Milano, Milano, Italy

Abstract

ABSTRACT A quantitative real-time PCR targeting the tnaA gene was studied to detect Escherichia coli and distinguish E. coli from Shigella spp. These microorganisms revealed high similarity in the molecular organization of the tna operon.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference21 articles.

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4. Brenner, D. J. 1984. Family I. Enterobacteriaceae, p. 408-420. In N. R. Krieg (ed.), Bergey's manual of systematic bacteriology,vol. 1. Williams & Wilkins, Baltimore, MD.

5. Cilia, V., B. Lafay, and R. Christen. 1996. Sequence heterogeneities among 16S ribosomal RNA sequences, and their effect on phylogenetic analysis at the species level. Mol. Biol. Evol.13:451-461.

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