Affiliation:
1. Venereal Disease Research Laboratory, National Communicable Disease Center, U.S. Department of Health, Education, and Welfare, Atlanta, Georgia 30333
Abstract
An automated, quantitative microhemagglutination assay for antibodies to
Treponema pallidum
was developed by using
T. pallidum
-sensitized erythrocytes and an automatic serial-dilution instrument. Reactivity was found in sera from 54 rabbits and 6 chimpanzees infected with
T. pallidum.
Reactivity was also found in sera from animals infected with
T. pertenue, T. carateum
, and
T. cuniculi
. No reactivity was found in sera from 75 normal rabbits or from 129 rabbits immunized with cultivatable treponemes or a variety of other bacteria. In approximately 3 min, 13 twofold serial dilutions of each of 8 preabsorbed sera and the addition of sensitized erythrocytes to each dilution were accomplished automatically. The automated assay can serve as a research tool in quantitating antibodies to pathogenic treponemes, and evaluation of its clinical usefulness seems warranted.
Publisher
American Society for Microbiology
Subject
General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine
Reference15 articles.
1. A fluorescent test for treponemal antibodies;Deacon W. E.;Proc. Soc. Exp. Biol. Med.,1957
2. Evaluation of an automatic diluting device for microbiological applications;Goss W. A.;Appl. Microbiol.,1968
3. An improved FTA test for syphilis, the absorption procedure (PTA-ABS);Hunter E. F.;Public Health Rep.,1964
4. False positive reactions in treponemal tests;Julian A. J.;Brit. J. Vener. Dis.,1963
5. Kuhn U. S. G. W. J. Brown and V. H. Falcone. 1968. Seroreactivity of nonhuman primates in treponemal and nontreponemal antigen tests for syphilis. World Health Organ. VDT/RES/68.137.
Cited by
8 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献