Serodiagnosis of infectious mononucleosis with a bovine erythrocyte glycoprotein

Author:

Fletcher M A,Klimas N G,Latif Z A,Caldwell K E

Abstract

A glycoprotein from bovine erythrocyte membrane was evaluated in two immunoassays as a reagent for the serodiagnosis of infectious mononucleosis (IM). We previously reported that a partially purified preparation of this glycoprotein, when attached to latex beads, agglutinated in the presence of IM heterophile antibody. In the present study, we used a highly purified form of the glycoprotein both as an agglutinating reagent, covalently bound to latex, and in a solid-phase sandwich-type radioimmunoassay (RIA) for IM antibody detection in a larger population of patients. We tested serum samples from college students with symptoms suggestive of IM with the latex reagent (143 samples) and with the RIA (245 samples). Correlation of these two tests, both with each other and with the classical differentially absorbed, agglutination tests for Paul-Bunnell antibody in IM sera, using fresh sheep or horse cells, was excellent (greater than 97% agreement). The new tests also corresponded in most cases with a rapid, unabsorbed preserved horse erythrocyte slide test. However, in this study of 245 samples, both apparent false-positives (5 samples) and apparent false-negatives (3 samples) were observed with this slide test. In conclusion, we found that the bovine glycoprotein as a reagent can facilitate the diagnosis of IM, giving results comparable to those with erythrocyte agglutination tests on differentially absorbed sera. The advantages are ease and speed of performance (latex test), potential for automation (RIA test), stability and uniformity of the glycoprotein reagent (latex and RIA tests), and most importantly, the ability to use unabsorbed sera (latex and RIA tests).

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference19 articles.

1. Hemolytic antibodies for sheep and ox erythrocyte in infectious mononucleosis;Bailey G. H.;J. Clin. Invest.,1935

2. Monospot: a differential slide test for infectious mononucleosis;Basson V.;J. Clin. Pathol.,1969

3. Immunochemical studies of infectious mononucleosis. X. Characterization of a glycoprotein from horse erythrocytes which reacts with Paul-Bunnell antibody;Caldwell K. E.;Mol. Immunol.,1982

4. Factors influencing the agglutinability of red cells. II. The agglutination of bovine red cells previously classified as "inagglutinable" by the building up of an "antiglobulin: globulin lattice" on the sensitized cells;Coombs R. R. A.;Br. J. Exp. Pathol.,1951

5. Blood group ADH-specific macroglycolipids of human erythrocytes: isolation in high yield from a crude membrane glycoprotein fraction;Dejter-Juszynski M.;Eur. J. Biochem.,1978

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3