Evaluation of OMNIgene Sputum and Ethanol Reagent for Preservation of Sputum Prior to Xpert and Culture Testing in Uganda-Reference-Cited by-同舟云学术

Evaluation of OMNIgene Sputum and Ethanol Reagent for Preservation of Sputum Prior to Xpert and Culture Testing in Uganda

Published:2019-12-23 Issue:1 Volume:58 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Ardizzoni Elisa¹,Orikiriza Patrick²³⁴,Ssuuna Charles⁴,Nyehangane Dan²,Gumsboga Mourad¹,Taremwa Ivan Mugisha²,Turyashemererwa Esther²,Mwanga-Amumpaire Juliet²,Langendorf Céline⁵,Bonnet Maryline²³⁶

Affiliation:

1. Institute of Tropical Medicine, Antwerp, Belgium

2. Epicentre Mbarara Research Centre, Mbarara, Uganda

3. Université de Montpellier, Montpellier, France

4. Department of Microbiology, Mbarara University of Science and Technology, Mbarara, Uganda

5. Epicentre Paris, Paris, France

6. Institute of Research and Development, Montpellier, France

Abstract

Xpert MTB/RIF (Xpert) and culture are the most reliable methods for tuberculosis diagnosis but are still poorly accessible in many low-resource countries. We aimed to assess the effects of OMNIgene Sputum (OM-S) and ethanol in preserving sputum for Xpert and OM-S for mycobacterial growth indicator tube (MGIT) testing over periods of 15 and 8 days, respectively. Sputum samples were collected from newly diagnosed smear-positive patients.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00810-19

Reference23 articles.

1. Global tuberculosis report 2018;WHO;Pharmacol Rep,2017

2. WHO. 2014. Xpert MTB/RIF implementation manual: technical and operational ‘how-to’: practical considerations. WHO Geneva Switzerland.

3. WHO. 2011. Automated real-time nucleic acid amplification technology for rapid and simultaneous detection of tuberculosis and rifampicin resistance: Xpert MTB/RIF. Policy statement. WHO, Geneva, Switzerland.

4. Cepheid. 2009. Operator manual test Xpert MTB/RIF. Cepheid Sunnyvale CA.

5. Ethanol fixation of sputum sediments for DNA-based detection of Mycobacterium tuberculosis

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