Conditions That Affect the Colorimetric Analysis of Lipopolysaccharide from Escherichia coli and Treponema pallidum

Abstract

Material extracted from the Nichols nonpathogenic strain of Treponema pallidum by phenol-water was analyzed by employing a recently reported colorimetric test for detection of lipopolysaccharide (LPS). The fraction isolated from T. pallidum , in combination with the reagent dye, absorbed maximally at a wavelength in the range reported to be positive for LPS. Comparison of this reaction to that of a commercial preparation of Escherichia coli LPS revealed that time and temperature of incubation of the LPS-dye complexes were important variables which had marked but different effects on the LPS of the two sources. However, with careful control of these parameters, concentration-dependent standard curves were established for LPS of both sources. Our results indicate the cell wall of T. pallidum is similar to that of gram-negative organisms.