Inhibition of β-Lactamases by β-Lactam Antibiotics

Author:

O'Callaghan Cynthia H.1,Morris A.1

Affiliation:

1. Glaxo Research Ltd., Greenford, England

Abstract

The inhibitory properties of a selected number of β-lactam antibiotics were studied, with the use of three distinct types of β-lactamases. The three enzymes were found to be distinguishable on the basis of their susceptibility to inhibition. Not one of the potential inhibitors tested was found to be a potent inhibitor of all three enzymes, but nafcillin possessed the broadest inhibitory activity. The enzyme isolated from Enterobacter cloacae was found to be the most susceptible. In some cases, the degree of inhibition varied with the time of incubation, and, depending upon the time chosen, widely different observations could be made. It is suggested that, in studies such as these, every consideration should be given to the period of incubation and to the concentration of inhibitor employed. Mixtures of inhibitor and cephaloridine did not always act synergistically against growing bacteria, and a number of reasons for failure are suggested.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Reference12 articles.

1. The biochemistry and function of 3-lactamase (penicillinase);Citri N.;Advan. Enzymol.,1966

2. Inhibition of the 3-lactamases of Escherichia coli and Klebsiella aerogenies by semi-synthetic penicillins;Cole M.;Biochem. J.,1972

3. The demonstration and sig-- nificance of synergism between 0-lactam antibiotics;Hamilton-Miller J. M. T.;J. Med. Microbiol.,1971

4. A comparative study of eight distinct 0-lactamases synthesised by Gram-negative bacteria;Jack G. W.;J. Gen. Microbiol.,1970

5. Inhibition of ,-lactamase decomposition of cephaloridine and cephalothin by other cephalosporins;O'Callaghan C. H.;Antimicrob. Ag. Chemother.,1967

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