Affiliation:
1. Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina
2. Biozentrum der Universität Basel, CH-4056 Basel, Switzerland
Abstract
ABSTRACT
The genomes of the hemiascomycetes
Saccharomyces cerevisiae
and
Ashbya gossypii
have been completely sequenced, allowing a comparative analysis of these two genomes, which reveals that a small number of genes appear to have entered these genomes as a result of horizontal gene transfer from bacterial sources. One potential case of horizontal gene transfer in
A. gossypii
and 10 potential cases in
S. cerevisiae
were identified, of which two were investigated further. One gene, encoding the enzyme dihydroorotate dehydrogenase (DHOD), is potentially a case of horizontal gene transfer, as shown by sequencing of this gene from additional bacterial and fungal species to generate sufficient data to construct a well-supported phylogeny. The DHOD-encoding gene found in
S. cerevisiae
,
URA1
(YKL216W), appears to have entered the
Saccharomycetaceae
after the divergence of the
S. cerevisiae
lineage from the
Candida albicans
lineage and possibly since the divergence from the
A. gossypii
lineage. This gene appears to have come from the
Lactobacillales
, and following its acquisition the endogenous eukaryotic DHOD gene was lost. It was also shown that the bacterially derived horizontally transferred DHOD is required for anaerobic synthesis of uracil in
S. cerevisiae.
The other gene discussed in detail is
BDS1
, an aryl- and alkyl-sulfatase gene of bacterial origin that we have shown allows utilization of sulfate from several organic sources. Among the eukaryotes, this gene is found in
S. cerevisiae
and
Saccharomyces bayanus
and appears to derive from the alpha-proteobacteria.
Publisher
American Society for Microbiology
Subject
Molecular Biology,General Medicine,Microbiology
Cited by
213 articles.
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