Affiliation:
1. Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan
Abstract
ABSTRACT
Vibrios are gram-negative γ-proteobacteria which are ubiquitous in marine and estuarine environments. Recently, we demonstrated that some, if not all,
Vibrio
species have two circular chromosomes. The whole genome sequence of
Vibrio cholerae
N16961 has been reported. In this study, we constructed a physical and genetic map of the genome of Kanagawa phenomenon-positive
Vibrio parahaemolyticus
strain KX-V237 and compared it with those of
V. parahaemolyticus
AQ4673 and
V. cholerae
N16961. The genome of KX-V237 comprised two circular chromosomes (3.3 and 1.9 Mb), similar to the structure of the AQ4673 genome. The relative positions of the genes on the genomes were well conserved in the two strains, but a large inversion on the large chromosomes, probably symmetric around the replication origin, was suggested. Although the sizes of the large chromosomes of KX-V237 and
V. cholerae
N16961 were similar, the sizes of the small chromosomes were very different. Unlike N16961, the superintegron of KX-V237 was located on the large chromosome. Comparison of the genetic maps of the chromosomes of KX-V237 and
V. cholerae
N16961 revealed that most of the open reading frames (ORFs) present on the large chromosome of the
V. cholerae
strain had homologues on the large chromosome of the
V. parahaemolyticus
strain and that most of the ORFs on the small chromosome of N16961 were present on the small chromosome of KX-V237. The difference in the orders of the ORFs on the chromosomes of N16961 and KX-V237 implies that numerous and frequent genetic exchanges have occurred intrachromosomally rather than interchromosomally.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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