Characterization of Amino Acid Substitutions in KdpA, the K + -Binding and -Translocating Subunit of the KdpFABC Complex of Escherichia coli-Reference-Cited by-同舟云学术

Characterization of Amino Acid Substitutions in KdpA, the K + -Binding and -Translocating Subunit of the KdpFABC Complex of Escherichia coli

Published:2002-10 Issue:19 Volume:184 Page:5491-5494
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

van der Laan Martin¹,Gaßel Michael¹,Altendorf Karlheinz¹

Affiliation:

1. Abteilung Mikrobiologie, Fachbereich Biologie/Chemie, Universität Osnabrück, D-49069 Osnabrück, Germany

Abstract

ABSTRACT When grown under K + limitation, Escherichia coli induces the K + -translocating KdpFABC complex. The stimulation of ATPase activity by NH 4 + ions was shown for the first time. Substitutions in KdpA, which is responsible for K + binding and translocation, revealed that enzyme complexes KdpA:G232A and KdpA:G232S have completely lost their cation selectivity.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.184.19.5491-5494.2002

Reference23 articles.

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