Examination of the Borrelia burgdorferi Transcriptome in Ixodes scapularis during Feeding-Reference-Cited by-同舟云学术

Examination of the Borrelia burgdorferi Transcriptome in Ixodes scapularis during Feeding

Published:2002-06 Issue:11 Volume:184 Page:3122-3125
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Narasimhan Sukanya¹,Santiago Felix¹,Koski Raymond A.²,Brei Brandon³,Anderson John F.⁴,Fish Durland³,Fikrig Erol¹

Affiliation:

1. Section of Rheumatology, Department of Internal Medicine

2. L Diagnostics, New Haven, Connecticut 06520

3. Department of Epidemiology and Public Health, Yale University School of Medicine

4. Department of Entomology, Connecticut Agricultural Experiment Station, New Haven, Connecticut 06504

Abstract

ABSTRACT Borrelia burgdorferi gene expression within the guts of engorging Ixodes scapularis ticks was examined by use of differential immunoscreening and differential expression with a customized amplified library. Fourteen chromosomal genes involved in energy metabolism, substrate transport, and signal transduction and 10 (4 chromosomal and 6 plasmid) genes encoding putative lipoproteins and periplasmic proteins were preferentially expressed in engorging ticks. These data demonstrate a new approach to the global analysis of B. burgdorferi genes that are preferentially expressed within the vector during feeding.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.184.11.3122-3125.2002

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