Molecular cloning with a pMEA300-derived shuttle vector and characterization of the Amycolatopsis methanolica prephenate dehydratase gene-Reference-Cited by-同舟云学术

Molecular cloning with a pMEA300-derived shuttle vector and characterization of the Amycolatopsis methanolica prephenate dehydratase gene

Published:1995-11 Issue:22 Volume:177 Page:6666-6669
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Vrijbloed J W¹,van Hylckama Vlieg J¹,van der Put N M¹,Hessels G I¹,Dijkhuizen L¹

Affiliation:

1. Department of Microbiology, University of Groningen, Haren, The Netherlands.

Abstract

An efficient restriction barrier for methylated DNA in the actinomycete Amycolatopsis methanolica could be avoided by using a nonmethylating Escherichia coli strain for DNA isolations. The A. methanolica prephenate dehydratase gene was cloned from a gene bank in a pMEA300-derived shuttle vector in E. coli and characterized.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.177.22.6666-6669.1995

Reference27 articles.

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