Affiliation:
1. California Institute of Technology, Pasadena 91125, USA.
Abstract
The DNA sequence for a 4.4-kb HindIII-XhoI Methylobacterium extorquens AM1 DNA fragment that is known to contain three genes (mxaAKL) involved in incorporation of calcium into methanol dehydrogenase (I. W. Richardson and C. Anthony, Biochem. J. 287:709-7115, 1992) was determined. Five complete open reading frames and two partial open reading frames were found, suggesting that this region contains previously unidentified genes. A combination of sequence analysis, mutant complementation data, and gene expression studies showed that these genes correspond to mxaSACKLDorf1. Of the three previously unidentified genes (mxaC, mxaD, and orf1), mutant complementation studies showed that mxaC is required for methanol oxidation, while the function of the other two genes is still unknown.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference48 articles.
1. Purification and properties of methanol dehydrogenase and aldehyde dehydrogenase from Methylobacillus glycogenes;Adachi O.;Agric. Biol. Chem.,1990
2. The moxFG region encodes four polypeptides in the methanol-oxidizing bacterium Methylobacterium sp. strain AM1;Anderson D. J.;J. Bacteriol.,1988
3. Nucleotide sequence of the Methylobacterium extorquens AM1 moxF and moxJ genes involved in methanol oxidation;Anderson D. J.;Gene,1990
4. Anthony C. 1982. The biochemistry of methylotrophs. Academic Press London.
5. The structure of bacterial quinoprotein dehydrogenases;Anthony C.;Int. J. Biochem.,1992
Cited by
25 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献