Affiliation:
1. Area of Microbiology, Faculty of Biology, University of León, Spain.
Abstract
A homolog of the Corynebacterium diphtheriae dtxR gene was isolated from Brevibacterium lactofermentum. The product of the B. lactofermentum dtxR gene was immunoreactive with polyclonal anti-DtxR antibodies and functioned as an iron-activated repressor capable of regulating the expression of beta-galactosidase from a diphtheria tox promoter/operator transcriptional fusion in recombinant Escherichia coli. The extents of induction by increasing concentrations of the chelator 2,2'-dipyridyl were identical in cells expressing DtxR from either C. diphtheriae or B. lactofermentum.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference15 articles.
1. Molecular cloning and DNA sequence analysis of a diphtheria tox iron-dependent regulatory element (dtxR) from Corynebacterium diphtheriae;Boyd J.;Proc. Natl. Acad. Sci. USA,1990
2. DNA sequencing and characterization of dtxR alleles from Corynebacterium diphtheriae PW8(~), 1030(~), and C7hm723(~);Boyd J. M.;J. Bacteriol.,1992
3. Characterization of an ironregulated promoter involved in desferrioxamine B synthesis in Streptomyces pilosus: repressor-binding site and homology to the diphtheria toxin gene promoter;Günter K.;J. Bacteriol.,1993
4. Martín J. F. R. F. Cadenas M. Malumbres L. M. Mateos C. Guerrero and J. A. Gil. 1990. Construction and utilization of promoter-probe and expression vectors in corynebacteria. Characterization of corynebacterial promoters p. 283-292. In H. Heslot J. Davies J. Florent L. Bobichon G. Durant and L. Penasse (ed.) Genetics of industrial microorganisms '90. Société Française de Microbiologie Strasbourg France.
5. Cloning systems in amino acid-producing corynebacteria;Martín J. F.;Bio/Technology,1987
Cited by
57 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献