Selective detection of Mycoplasma hyorhinis using cloned genomic DNA fragments

Abstract

A 13-kilobase DNA fragment from a genomic library of Mycoplasma hyorhinis demonstrated specific Southern hybridization and dot hybridization when tested against a group of different mycoplasmas. This probe selectively recognized M. hyorhinis sequences in purified DNA, broth-grown organisms, and infected cell cultures, providing a direct method for differential detection of this species.