Author:
de la Maza L M,Peterson E M,Goebel J M,Fennie C W,Czarniecki C W
Abstract
The yield of infectious Chlamydia trachomatis was analyzed in human (HeLa) and mouse (McCoy) cell lines treated with the human interferon (IFN) subtypes IFN-alpha A and IFN-alpha D, with their hybrids [IFN-alpha AD (BglII), IFN-alpha AD (PvuII), and IFN-alpha DA (BglII)] constructed in vitro from their expression plasmids, or with IFN-beta 1 or buffy coat IFN. In HeLa cells, a significant inhibition of Chlamydia infectivity was obtained with IFN-alpha D, IFN-alpha DA (BglII), and buffy coat IFN. In McCoy cells, IFN-alpha AD (BglII) and IFN-alpha AD (PvuII) induced a strong degree of inhibition of Chlamydia infectivity. In McCoy cells, there was a correlation among the antichlamydial, antiviral, and antiproliferative activities of the different IFNs tested. In HeLa cells, however, the ability of a particular IFN subtype to inhibit Chlamydia infectivity did not always correlate with its inhibitory effects on encephalomyocarditis virus replication or with its antiproliferative activity.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference23 articles.
1. Species specificity;Bucknall R. A.;Nature (London),1967
2. Immunological and karyological criteria for identification of cell lines;Defendi V.;J. Natl. Cancer Inst.,1960
3. Ultrastructural analysis of the growth cycle of Chlamydia trachomatis in mouse cells treated with recombinant human a-interferons;de la Maza L. M.;Exp. Mol. Pathol.,1984
4. Antiproliferative activities of bacterial-derived human alpha and beta interferons on human and mouse tumor cells;de la Maza L. M.;J. Interferon Res.,1983
5. A human interferon that crosses the species line;Desmyter J.;Proc. Natl. Acad. Sci. U.S.A.,1968
Cited by
42 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献