Optimization of RNA Extraction for PCR Quantification of Aromatic Compound Degradation Genes-Reference-Cited by-同舟云学术

Optimization of RNA Extraction for PCR Quantification of Aromatic Compound Degradation Genes

Published:2010-02-15 Issue:4 Volume:76 Page:1282-1284
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Kong Weidong¹,Nakatsu Cindy H.¹

Affiliation:

1. Department of Agronomy, Purdue University, West Lafayette, Indiana

Abstract

ABSTRACT Seven different bacterial strains and primer sets and a mixed community were used to evaluate the use of reverse transcriptase quantitative PCR (Q-PCR) and Q-PCR of oxygenase genes to assess various approaches for monitoring the bioremediation of polluted sites. Differences in maximum activity were seen when different RNA extraction kits were compared.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.01939-09

Reference18 articles.

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