Affiliation:
1. Division of General Microbiology, Department of Biosciences, FIN-00014 University of Helsinki, Finland
Abstract
ABSTRACT
A multivalent, bifunctional flagellum carrying two different adhesive peptides in separate flagellin subunits within a filament was constructed in
Escherichia coli
. The inserted peptides were the fibronectin-binding 115-mer D repeat region of
Staphylococcus aureus
and the 302-mer collagen-binding region of YadA of
Yersinia enterocolitica
. Western blotting, immunoelectron microscopy, and adhesion tests with hybrid flagella from an in
trans
-complemented Δ
fliC E. coli
strain showed that individual filaments consisted of both recombinant flagellins.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
13 articles.
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