Purification and characterization of herpes simplex virus type 1 alkaline exonuclease expressed in Escherichia coli-Reference-Cited by-同舟云学术

Purification and characterization of herpes simplex virus type 1 alkaline exonuclease expressed in Escherichia coli

Published:1996-03 Issue:3 Volume:70 Page:2008-2013
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Bronstein J C¹,Weber P C¹

Affiliation:

1. Infectious Diseases Section, Parke-Davis Pharmaceutical Research, Division of Warner-Lambert Company, Ann Arbor, Michigan 48105, USA.

Abstract

The alkaline exonuclease (AE) encoded by the herpes simplex virus type 1 (HSV-1) UL12 open reading frame was inducibly expressed in Escherichia coli and purified without the use of chromatographic separation. This recombinant AE was found to exhibit the same biochemical properties as the virus-encoded protein and was used to confirm the existence of a weak endonucleolytic activity in the enzyme. Antisera raised against the recombinant protein recognized several forms of the AE in HSV-1-infected cells. This expression and purification strategy will provide an economical and easily accessible alternative source of HSV-1 AE for future in vitro studies.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jvi.70.3.2008-2013.1996

Reference16 articles.

1. Herpes simplex virus non-structural proteins. IV. Purification of the virusinduced deoxyribonuclease and characterization of the enzyme using monoclonal antibodies;Banks L.;J. Gen. Virol.,1983

2. The characterization of the EBV alkaline deoxyribonuclease cloned and expressed in E. coli;Baylis S. A.;Nucleic Acids Res.,1989

3. Bronstein J. C. and P. C. Weber. Unpublished data.

4. Use of bacterially-expressed antigen for detection of antibodies to the EBVspecific deoxyribonuclease in sera from patients with nasopharyngeal carcinoma;Chen J. Y.;J. Virol. Methods,1993

5. High-resolution characterization of herpes simplex virus type 1 transcripts encoding alkaline exonuclease and a 50,000-Dalton protein tentatively identified as a capsid protein;Costa R. H.;J. Virol.,1983

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