Affiliation:
1. Department of Bacteriology, University of Wisconsin—Madison, Madison, Wisconsin 53706
Abstract
ABSTRACT
The bacterium
Vibrio natriegens
can double with a generation time of less than 10 min (R. G. Eagon, J. Bacteriol. 83:736-737, 1962), a growth rate that requires an extremely high rate of protein synthesis. We show here that
V. natriegens
' high potential for protein synthesis results from an increase in ribosome numbers with increasing growth rate, as has been found for other bacteria. We show that
V. natriegens
contains a large number of rRNA operons, and its rRNA promoters are extremely strong. The
V. natriegens
rRNA core promoters are at least as active in vitro as
Escherichia coli
rRNA core promoters with either
E. coli
RNA polymerase (RNAP) or
V. natriegens
RNAP, and they are activated by UP elements, as in
E. coli
. In addition, the
E. coli
transcription factor Fis activated
V. natriegens rrn
P1 promoters in vitro. We conclude that the high capacity for ribosome synthesis in
V. natriegens
results from a high capacity for rRNA transcription, and the high capacity for rRNA transcription results, at least in part, from the same factors that contribute most to high rates of rRNA transcription in
E. coli
, i.e., high gene dose and strong activation by UP elements and Fis.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
99 articles.
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