Affiliation:
1. Department of Genomics and Strain Development, Chr. Hansen A/S., DK-2970 Hørsholm, Denmark
Abstract
ABSTRACT
A plasmid-borne diacetyl (acetoin) reductase (
butA
) from
Leuconostoc pseudomesenteroides
CHCC2114 was sequenced and cloned. Nucleotide sequence analysis revealed an open reading frame encoding a protein of 257 amino acids which had high identity at the amino acid level to diacetyl (acetoin) reductases reported previously. Downstream of the
butA
gene of
L. pseudomesenteroides
, but coding in the opposite orientation, a putative DNA recombinase was identified. A two-step PCR approach was used to construct FPR02, a
butA
mutant of the wild-type strain, CHCC2114. FPR02 had significantly reduced diacetyl (acetoin) reductase activity with NADH as coenzyme, but not with NADPH as coenzyme, suggesting the presence of another diacetyl (acetoin)-reducing activity in
L. pseudomesenteroides
. Plasmid-curing experiments demonstrated that the
butA
gene is carried on a 20-kb plasmid in
L. pseudomesenteroides
.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
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