Interaction of pH and NaCl on Enumeration of Heat-Stressed Staphylococcus aureus

Author:

Smolka Lawrence R.12,Nelson F. Eugene12,Kelley Lee M.12

Affiliation:

1. Department of Microbiology and Medical Technology, The University of Arizona, Tucson, Arizona 85721

2. Department of Dairy and Food Sciences, The University of Arizona, Tucson, Arizona 85721

Abstract

The effect of pH level and NaCl concentrations, alone and in combination, on the enumeration of unstressed and heat-stressed cells of three strains of Staphylococcus aureus was determined. A definite narrowing of the optimum pH range for enumeration of both unstressed and heat-stressed cells was observed as the NaCl concentration was increased from 0.0 to 7.5%. Counts of unstressed cells diminished only slightly with increases in NaCl, whereas heat-stressed cells showed a marked sensitivity to NaCl concentrations of 4% and above, regardless of the pH level. Because of this sensitivity to NaCl, recoveries were far poorer than with unstressed cells at NaCl concentrations of 4% and above.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Reference17 articles.

1. American Public Health Association. 1972. Standard methods for the examination of dairy products 13th ed. American Public Health Association Inc. Washington D.C.

2. Effect of sodium chloride concentration in an agar media on the growth of heat-shocked Staphylococcus aureus;Busta F. F.;Appl. Microbiol.,1963

3. The significance of sodium chloride in studies of staphylococci;Chapman G. H.;J. Bacteriol.,1946

4. An improved medium for the isolation and testing of food-poisoning staphylococci;Chapman G. H.;Food Res.,1948

5. Effect of sodium chloride and pH on enterotoxin C production;Genigeorgis C.;Appl. Microbiol.,1971

Cited by 3 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Sublethal injury, pathogen virulence and adaptation;Understanding Pathogen Behaviour;2005

2. Effects of Blood on Blood Culture Medium;Journal of Clinical Microbiology;1979-10

3. Injury to Staphylococcus aureus during sausage fermentation;Applied and Environmental Microbiology;1978-12

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