Dot Blot Assay for Detection of Antidiacyltrehalose Antibodies in Tuberculous Patients-Reference-Cited by-同舟云学术

Dot Blot Assay for Detection of Antidiacyltrehalose Antibodies in Tuberculous Patients

Published:1999-09 Issue:5 Volume:6 Page:686-689
ISSN:1071-412X
Container-title:Clinical Diagnostic Laboratory Immunology
language:en
Short-container-title:Clin Diagn Lab Immunol

Author:

Vera-Cabrera Lucio¹²,Rendon Adrian³,Diaz-Rodriguez Manuel³,Handzel Vera²,Laszlo Adalbert²

Affiliation:

1. Servicios de Dermatologı́a1 and

2. National Reference Centre for Tuberculosis, Laboratory Centre for Disease Control, Health Canada, Ottawa, Canada K1A OL22

3. Neumologı́a,3 Hospital Universitario “Dr. José Eleuterio González,” Monterrey, Nuevo León, México, and

Abstract

ABSTRACT A simple dot blot test with diacyltrehalose (DAT) as the antigen was developed to detect anti-DAT antibodies in tuberculous patients. To enhance antigen-antibody reaction detection, rabbit serum raised against human immunoglobulins was used prior to incubation with a protein A-colloidal gold complex. With the dot blot system, it was possible to obtain a sensitivity similar to that of enzyme-linked immunosorbent assay (ELISA) and a specificity of 97.14%, versus a specificity of 94.29% by the ELISA. We conclude that this simple and fast assay could be used in places where ELISA equipment is not easy available and that it might also be applicable with other Mycobacterium tuberculosis immunogenic antigens.

Publisher

American Society for Microbiology

Subject

Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy

Link

https://journals.asm.org/doi/pdf/10.1128/CDLI.6.5.686-689.1999

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