A Reverse-Sandwich Enzyme-Linked Immunosorbent Assay for Verocytotoxin 1 and 2 Antibodies in Human and Bovine Sera-Reference-Cited by-同舟云学术

A Reverse-Sandwich Enzyme-Linked Immunosorbent Assay for Verocytotoxin 1 and 2 Antibodies in Human and Bovine Sera

Published:1999-09 Issue:5 Volume:6 Page:701-704
ISSN:1071-412X
Container-title:Clinical Diagnostic Laboratory Immunology
language:en
Short-container-title:Clin Diagn Lab Immunol

Author:

Miyazawa H.¹,Bannai H.¹,Yanase T.²,Morita C.²,Satoh S.³,Sugiyama J.³,Taniguchi S.⁴,Inouye S.⁴

Affiliation:

1. Department of Medical Technology, Kyorin University School of Health Sciences, Hachioji-shi,1 and

2. Department of Veterinary Public Health, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu-shi, Hokkaido,2 and

3. Denka Seiken Co., Gosen-shi, Niigata,3Japan

4. Infectious Disease Surveillance Center, National Institute of Infectious Diseases, Shinjuku-ku,4 Tokyo,

Abstract

ABSTRACT A reverse-sandwich enzyme-linked immunosorbent assay (ELISA), in which an antibody is sandwiched by antigens, was established for the titration of antibodies to verocytotoxins (VT) in human and animal sera. This assay has two advantages over a conventional indirect ELISA: (i) higher specificity and sensitivity and (ii) the ability to comparably titrate antibodies from different species. The VT1 (Shiga-like toxin 1) antibody-positive rates were 5% in 202 normal adult humans and 99% in 93 normal cattle at a dairy farm. This ELISA is most suitable for seroepidemiologic studies of infections with VT-producing Escherichia coli in humans and various animal species.

Publisher

American Society for Microbiology

Subject

Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy

Link

https://journals.asm.org/doi/pdf/10.1128/CDLI.6.5.701-704.1999

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