Author:
Von Tersch M A,Carlton B C
Abstract
A host-vector system was developed for molecular cloning in Bacillus megaterium and used to clone the structural and immunity genes for megacins A-216 and A-19213. Recombinant clones that expressed immunity only or both immunity to and production of each megacin were obtained. Restriction mapping of native megacinogenic plasmids and recombinant clones was used to construct physical and genetic maps of megacinogenic plasmids pBM309 and pBM113. Limited sequence homology between pBM309 and pBM113 was detected by Southern blot hybridization and was mapped to, at most, a 6.4-kilobase-pair region of pBM309 and a 6.1-kilobase-pair region of pBM113.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference20 articles.
1. Bacteriocin and antibiotic resistance plasmids in Bacillus cereus and Bacillus subtilis;Bernhard K.;J. Bacteriol.,1978
2. Plasmid-mediated transformation in Bacillus megaterium;Brown B. J.;J. Bacteriol.,1980
3. Dubnau D. T. Gryczan S. Contente and A. G. Shivakumar. 1980. Molecular cloning in Bacillus subtilis p. 115-131. In J. K. Setlow and A. Hollaender (ed.) Genetic engineering vol. 2. Plenum Press New York.
4. Characterization of Staphylococcus aureus plasmids introduced by transformation into Bacillus subtilis;Gryczan T. J.;J. Bacteriol.,1978
5. Some properties of a new bacteriocin formed by Bacillus megaterium;Holland I. B.;J. Gen. Microbiol.,1964
Cited by
17 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献