Enrichment Broth Improved Detection of Extended-Spectrum-Beta-Lactamase-Producing Bacteria in Throat and Rectal Surveillance Cultures of Samples from Patients in Intensive Care Units

Author:

Murk Jean-Luc A. N.1,Heddema Edou R.2,Hess Dorine L. J.1,Bogaards Johannes A.3,Vandenbroucke-Grauls Christina M. J. E.1,Debets-Ossenkopp Yvette J.1

Affiliation:

1. VU University Medical Center, Medical Microbiology and Infection Control, Amsterdam, The Netherlands

2. Maaslandhospital, Medical Microbiology, Sittard, The Netherlands

3. VU University Medical Center, Epidemiology and Biostatistics, Amsterdam, The Netherlands

Abstract

ABSTRACT We evaluated the use of a trypticase soy broth (TSB) for improving detection of extended-spectrum-beta-lactamase-producing (ESBL + ) bacteria. Preenrichment of throat and rectal swabs in TSB prior to inoculation on solid medium doubled the number of ESBL + bacteria detected in samples obtained from patients in our intensive care unit.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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