Direct PCR on Tissue Samples To Detect Mycobacterium tuberculosis Complex: an Alternative to the Bacteriological Culture-Reference-Cited by-同舟云学术

Direct PCR on Tissue Samples To Detect Mycobacterium tuberculosis Complex: an Alternative to the Bacteriological Culture

Published:2021-01-21 Issue:2 Volume:59 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Lorente-Leal V.¹²,Liandris E.¹,Pacciarini M.³,Botelho A.⁴^ORCID,Kenny K.⁵,Loyo B.⁶,Fernández R.⁷,Bezos J.¹²,Domínguez L.¹²,de Juan L.¹²,Romero B.¹^ORCID

Affiliation:

1. VISAVET Health Surveillance Center, Complutense University of Madrid, Madrid, Spain

2. Veterinary Faculty, Animal Health Department, Complutense University of Madrid, Madrid, Spain

3. Istituto Zooprofilattico Sperimentale of Lombardia and Emilia-Romagna (IZSLER), National Reference Centre for Bovine tuberculosis, Brescia, Italy

4. IP National Institute for Agrarian and Veterinary Research (INIAV), Oeiras, Portugal

5. Central Veterinary Research Laboratory (CVRL), Department of Agriculture, Food and the Marine, Celbridge, Ireland

6. Laboratorio Regional de Sanidad Animal, Servicio de Sanidad Animal, Dirección General de Producción Agropecuaria, Consejería de Agricultura, Ganadería y Desarrollo Rural de la Junta de Castilla y León, Villaquilambre, Spain

7. Laboratorio Central de Sanidad Animal. Ministerio de Agricultura, Pesca y Alimentación, Granada, Spain

Abstract

Bovine tuberculosis (bTB) is an ongoing issue in several countries within the European Union. Microbiological culture is the official confirmation technique for the presence of Mycobacterium tuberculosis complex (MTBC) members in bovine tissues, but several methodological issues, such as moderate sensitivity and long incubation times, require the development of more sensitive and rapid techniques.

Funder

Banco Santander

European Union Reference Laboratory for Bovine Tuberculosis

Area de Ganaderia de la Comunidad de Madrid

Ministerio de Agricultura, Pesca y Alimentación

Universidad Complutense de Madrid

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01404-20

Reference56 articles.

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3. The European Union One Health 2018 Zoonoses Report

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5. Primary isolation of Mycobacterium bovis from bovine tissues: Conditions for maximising the number of positive cultures

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