Affiliation:
1. Department of Microbiology and Immunology, University of Southern California, Los Angeles, California 90007
2. School of Dentistry, Los Angeles, California 90007
Abstract
We developed a method for determining the specificity of plasma cells for bacterial antigens in situ. The method consists of applying rhodamine-labeled whole bacteria to frozen sections of tissue in which inflammation had been induced by injection of the same bacterial strain. After appropriate washings, the tissue sections were briefly treated with a fluorescein-labeled antiserum to endogenous immunoglobulins to detect plasma cells. Plasma cells which could recognize bacterial somatic antigens appeared as clumps of orange-fluorescing bacteria overlying a green-fluorescing cell. The fidelity of the procedure was determined by performing several competition experiments, which showed that pretreatment of the inflamed tissue sections with homologous unlabeled bacteria (i.e., the same bacteria used to induce the tissue inflammation) decreased binding of the labeled bacteria to a significant extent. Pretreatment of the inflamed tissue sections with heterologous unlabeled bacteria, however, did not significantly decrease binding of the labeled bacterium. Additionally, binding of rhodamine-labeled heterologous bacteria to the tissue sections was insignificant. In further experiments, we demonstrated that there was no natural affinity between the bacterial strains employed and lymphocytes taken from the strain of rabbit used and that pretreatment of the sections with anti-immunoglobulin abrogated the binding of the bacteria to which the animal had been specifically sensitized.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference7 articles.
1. Cell-mediated immune responses in oral disease: a review;Lehner T.;J. Oral Path.,1972
2. The identification ofsix mouse Iymphocyte subpopulations by their natural binding of bacteria;Mayer E. P.;J. Immunol.,1978
3. Periodontal disease: a model for the study of inflammation;Mergenhagen S. E.;J. Infect. Dis.,1971
4. Presence of antibodies specific to cartilage-type collagen in rheumatoid synovial tissue;Mestecky J.;Clin. Exp. Immunol.,1975
5. The role of immunology in periodontal disease;Nisengard R. J.;J. Periodontol.,1977
Cited by
4 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献