Development of a Standardized Sanger-Based Method for Partial Sequencing and Genotyping of Dengue Viruses

Author:

Santiago Gilberto A.1,González Glenda L.1,Cruz-López Fabiola1,Muñoz-Jordan Jorge L.1

Affiliation:

1. Centers for Disease Control and Prevention, National Centers for Emerging and Zoonotic Infectious Diseases, Division of Vector Borne Diseases, Dengue Branch, San Juan, Puerto Rico

Abstract

The global expansion of dengue viruses (DENV-1 to DENV-4) has contributed to the divergence, transmission, and establishment of genetic lineages of epidemiological concern; however, tracking the phylogenetic relationships of these virus is not always possible due to the inability of standardized sequencing procedures in resource-limited public health laboratories. Consequently, public genomic data banks contain inadequate representation of geographical regions and historical periods.

Funder

HHS | Centers for Disease Control and Prevention

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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