Evaluation of a Combined Multilocus Sequence Typing and Whole-Genome Sequencing Two-Step Algorithm for Routine Typing of Clostridioides difficile-Reference-Cited by-同舟云学术

Evaluation of a Combined Multilocus Sequence Typing and Whole-Genome Sequencing Two-Step Algorithm for Routine Typing of Clostridioides difficile

Published:2021-01-21 Issue:2 Volume:59 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Kamboj Mini¹²,McMillen Tracy³,Syed Mustafa⁴,Chow Hoi Yan²,Jani Krupa³,Aslam Anoshe²,Brite Jennifer²,Fanelli Brian⁵,Hasan Nur A.⁵^ORCID,Dadlani Manoj⁵,Westblade Lars⁶,Zehir Ahmet⁴,Simon Matthew⁷,Babady N. Esther¹³^ORCID

Affiliation:

1. Infectious Disease Service, Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, New York, USA

2. Infection Control Service, Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, New York, USA

3. Clinical Microbiology Service, Department of Laboratory Medicine, Memorial Sloan Kettering Cancer Center, New York, New York, USA

4. Diagnostic Molecular Pathology Service, Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, New York, USA

5. CosmosID Inc., Rockville, Maryland, USA

6. Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, New York, USA

7. Division of Infectious Diseases, Department of Medicine, Weill Cornell Medicine, New York, New York, USA

Abstract

Multilocus sequence typing (MLST) is a low-resolution but rapid genotyping method for Clostridioides difficile . Whole-genome sequencing (WGS) has emerged as the new gold standard for C. difficile typing, but cost and lack of standardization still limit broad utilization. In this study, we evaluated the potential to combine the portability of MLST with the increased resolution of WGS for a cost-saving approach to routine C. difficile typing.

Funder

HHS | NIH | National Cancer Institute

HHS | National Institutes of Health

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01955-20

Reference29 articles.

1. Burden ofClostridium difficileInfection in the United States

2. Emergence of Clostridium difficile-associated disease in North America and Europe