Affiliation:
1. Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA.
Abstract
The antimicrobial, immunomodulatory, and cell growth-regulatory activities of the interferons are mediated by interferon-inducible proteins. One of these is p202, a nuclear protein that is encoded by the Ifi 202 gene from the interferon-activatable gene 200 cluster. Overexpression of p202 in transfected cells slows down cell proliferation. As shown earlier, p202 binds to the hypophosphorylated form of the retinoblastoma susceptibility protein. Here we report that p202 inhibits the activities of the NF-kappa B and the AP-1 enhancers both in transiently transfected cells and in transfected stable cell lines overexpressing p202. Furthermore, p202 binds the NF-kappa B p50 and p65 and the AP-1 c-Fos and c-Jun transcription factors in vitro and in vivo. NF-kappa B, c-Fos, and c-Jun participate in the transcription of various cellular and viral genes, and thus p202 can modulate the expression of these genes in response to interferons.
Publisher
American Society for Microbiology
Subject
Cell Biology,Molecular Biology
Reference76 articles.
1. Transcriptional regulation by Fos and Jun in vitro: interaction among multiple activator and regulatory domains;Abate C.;Mol. Cell. Biol.,1991
2. Expression and purification of the leucine zipper and DNA-binding domains of Fos and Jun: both Fos and Jun contact DNA directly;Abate C.;Proc. Natl. Acad. Sci. USA,1990
3. Phorbol ester-inducible genes contain a common cis element recognized by a TPA-modulated trans-acting factor;Angel P.;Cell,1987
4. The role of Jun, Fos and the AP-1 complex in cell proliferation and transformation;Angel P.;Biochim. Biophys. Acta,1991
5. Function and activation of NF-kappa-B in the immune system. Annu;Baeuerle P. A.;Rev. Immunol.,1994
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