Correlation between Bioassay and Protein Misfolding Cyclic Amplification for Variant Creutzfeldt-Jakob Disease Decontamination Studies

Author:

Bélondrade Maxime1,Jas-Duval Christelle12,Nicot Simon1,Bruyère-Ostells Lilian1,Mayran Charly1,Herzog Laetitia2,Reine Fabienne2,Torres Juan Maria3,Fournier-Wirth Chantal1,Béringue Vincent2ORCID,Lehmann Sylvain4,Bougard Daisy1ORCID

Affiliation:

1. Pathogenesis and Control of Chronic Infections, Etablissement Français du Sang, INSERM, Université de Montpellier, Montpellier, France

2. VIM INRA, Université Paris-Saclay, Jouy-en-Josas, France

3. Centro de Investigación en Sanidad Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (CISA-INIA), Madrid, Spain

4. CHRU de Montpellier and Université de Montpellier, IRMB, INSERM U1183, Laboratoire de Biochimie Protéomique Clinique, Montpellier, France

Abstract

Creutzfeldt-Jakob diseases are neurodegenerative disorders for which transmission linked to medical procedures have been reported in hundreds of patients. As prion diseases, they are characterized by an unusual resistance to conventional decontamination processes. Moreover, their large tissue distribution and the ability of prions to attach to many surfaces raised the risk of transmission in health care facilities. It is therefore of major importance that decontamination procedures applied to medical devices before their reprocessing are thoroughly validated for prion inactivation. We previously described an in vitro assay, which allowed us to classify accurately prion decontamination treatments according to their efficacy on variant Creutzfeldt-Jakob disease. The significance of this study is in demonstrating the concordance between previous in vitro results and infectivity studies in transgenic mice. Furthermore, commercial reagents currently used in hospitals were tested by both protocols, and we observed that most of them were ineffective on human prions.

Funder

Agence Nationale de Sécurité du Médicament et des Produits de Santé

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

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