Extracellular Vesicle-Mediated RNA Release in Histoplasma capsulatum

Abstract

Extracellular vesicles (EVs) play important roles in cellular communication and pathogenesis. The RNA molecules in EVs have been implicated in a variety of processes. EV-associated RNA classes have recently been described in pathogenic fungi; however, only a few reports of studies describing the RNAs in fungal EVs are available. Improved knowledge of EV-associated RNA will contribute to the understanding of their role during infection. In this study, we described the RNA content in EVs produced by two isolates of Histoplasma capsulatum . Our results add this important pathogen to the current short list of fungal species with the ability to use EVs for the extracellular release of RNA.