Cloning of genes that complement yeast hexokinase and glucokinase mutants

Author:

Walsh R B,Kawasaki G,Fraenkel D G

Abstract

Genes complementing the glucose-negative fructose-negative Saccharomyces cerevisiae triple mutant strain (hxkl hxk2 glk1), which lacks hexokinase PI, hexokinase PII, and glucokinase, were obtained from a pool of yeast DNA in the multicopy plasmid YEp13.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference11 articles.

1. Involvement of kinases in fructose and glucose uptake by Saccharomyces cerevisiae;Bluson L.;Proc. Natl. Acad. Sci. U.S.A.,1983

2. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system;Bolivar F.;Gene,1977

3. Glycolysis mutants in Saccharomyces cerevisiae;Clifton D.;Genetics,1978

4. Genetic evidence for a role of hexokinase isozyme Pll in carbon catabolite repression in Saccharomyces cerevisiae;Entlan K.;J. Biol. Chem.,1981

5. Yeast hexokinase mutants;Gancedo J.;J. Biol. Chem.,1977

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