Gas-liquid chromatography technique for detection of hippurate hydrolysis and conversion of fumarate to succinate by microorganisms

Author:

Kodaka H,Lombard G L,Dowell V R

Abstract

A gas-liquid chromatography technique which allows simultaneous detection of hippuric acid (N-benzoylglycine) hydrolysis and conversion of fumaric acid to succinic acid by microorganisms uses a new medium, hippurate-formate-fumarate broth, and a gas chromatograph equipped with a thermal conductivity detector. This technique gave more reproducible results than other tests used in the study for detecting hippurate hydrolysis and also gave consistent results in detecting succinic acid produced from utilization of fumaric acid.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference12 articles.

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2. Cultural and physiological characteristics and antimicrobial susceptibility of Clostridium botulinum isolates from foodborne and infant botulism cases;Dezfulian M.;J. Clin. Microbiol.,1980

3. DoweDl V. R. Jr. G. L. Lombard F. S. Thompson and A. Y. Armfield. 1977. Media for isolation characterization and identification of obligately anaerobic bacteria. Centers for Disease Control Atlanta Ga.

4. Rapid, colorimetric test for the determination of hippurate hydrolysis of group B Streptococcus;Edberg S. C.;J. Clin. Microbiol.,1976

5. Differentiation of A. aerogenes and A. cloacae on the basis of the hydrolysis of sodium hippurate;Hajna A. A.;Am. J. Hyg.,1934

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