Induction of Xylose Reductase and Xylitol Dehydrogenase Activities in Pachysolen tannophilus and Pichia stipitis on Mixed Sugars

Author:

Bicho Paul A.1,Runnals P. Lynn1,Cunningham J. Douglas1,Lee Hung1

Affiliation:

1. Department of Environmental Biology, University of Guelph, Guelph, Ontario, Canada N1G 2W1

Abstract

The induction of xylose reductase and xylitol dehydrogenase activities on mixed sugars was investigated in the yeasts Pachysolen tannophilus and Pichia stipitis . Enzyme activities induced on d -xylose served as the controls. In both yeasts, d -glucose, d -mannose, and 2-deoxyglucose inhibited enzyme induction by d -xylose to various degrees. Cellobiose, l -arabinose, and d -galactose were not inhibitory. In liquid batch culture, P. tannophilus utilized d -glucose and d -mannose rapidly and preferentially over d -xylose, while d -galactose consumption was poor and lagged behind that of the pentose sugar. In P. stipitis , all three hexoses were used preferentially over d -xylose. The results showed that the repressibility of xylose reductase and xylitol dehydrogenase may limit the potential of yeast fermentation of pentose sugars in hydrolysates of lignocellulosic substrates.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference25 articles.

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2. Xylose fermentation by yeasts: comparison of Pachysolen tannophilus and Pichia stipitis;Dellweg H.;Biotechnol. Lett.,1984

3. Fermentation of wheat straw hemicelluloses to ethanol by Pachysolen tannophilus;Detroy R. W.;Biotechnol. Bioeng. Symp.,1982

4. Xylose metabolism in Pachysolen tannophilus: purification and properties of xylose reductase;Ditzelmuller G.;Can. J. Microbiol.,1984

5. The fermentation of hexose and pentose sugars by Candida shehatae and Pichia stipitis;du Preez J. C.;Appl. Microbiol. Biotechnol.,1986

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