Validation and Implementation of a Diagnostic Algorithm for DNA Detection of Bordetella pertussis, B. parapertussis, and B. holmesii in a Pediatric Referral Hospital in Barcelona, Spain-Reference-Cited by-同舟云学术

Validation and Implementation of a Diagnostic Algorithm for DNA Detection of Bordetella pertussis, B. parapertussis, and B. holmesii in a Pediatric Referral Hospital in Barcelona, Spain

Published:2019-01 Issue:1 Volume:57 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Valero-Rello Ana¹,Henares Desiree¹²,Acosta Lesly³⁴,Jane Mireia²⁴,Jordan Iolanda¹²⁵,Godoy Pere²⁶,Muñoz-Almagro Carmen¹²⁷

Affiliation:

1. Institut de Recerca Sant Joan de Déu, Hospital Sant Joan de Déu, Barcelona, Spain

2. Network of Epidemiology and Public Health, CIBERESP, Madrid, Spain

3. Department of Statistics and Operations Research, Universitat Politecnica de Catalunya (BarcelonaTech), Barcelona, Spain

4. Subdirecció General de Vigilància i Resposta a Alertas de Salut Pública (ASPCAT), Barcelona, Spain

5. Pediatric Intensive Care Department, Hospital Sant Joan de Déu, Universitat de Barcelona, Barcelona, Spain

6. Institut de Recerca Biomèdica de Lleida, Lleida, Spain

7. School of Medicine, Universitat Internacional de Catalunya, Barcelona, Spain

Abstract

This study aimed to validate a comprehensive diagnostic protocol based on real-time PCR for the rapid detection and identification of Bordetella pertussis , Bordetella parapertussis , and Bordetella holmesii , as well as its implementation in the diagnostic routine of a reference children’s hospital. The new algorithm included a triplex quantitative PCR (qPCR) targeting IS 481 gene (in B. pertussis , B. holmesii , and some Bordetella bronchiseptica strains), pIS 1001 ( B. parapertussis -specific) and rnase P as the human internal control.

Funder

CIBER de Epidemiologia y Salud Publica

European Centre for Disease Prevention and Control

MINECO | Instituto de Salud Carlos III

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01231-18

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