Pyrosequencing for Rapid Molecular Detection of Rifampin and Isoniazid Resistance in Mycobacterium tuberculosis Strains and Clinical Specimens-Reference-Cited by-同舟云学术

Pyrosequencing for Rapid Molecular Detection of Rifampin and Isoniazid Resistance in Mycobacterium tuberculosis Strains and Clinical Specimens

Published:2011-10 Issue:10 Volume:49 Page:3683-3686
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

García-Sierra N.¹²,Lacoma A.¹²,Prat C.¹²,Haba L.¹,Maldonado J.³,Ruiz-Manzano J.⁴²,Gavin P.²⁵⁶,Samper S.²⁵⁶,Ausina V.¹²,Domínguez J.¹²

Affiliation:

1. Servei de Microbiologia, Hospital Universitari Germans Trias i Pujol, Institut d'Investigació Germans Trias i Pujol, Universitat Autònoma de Barcelona, Badalona, Spain

2. CIBER Enfermedades Respiratorias, Badalona, Spain

3. Serveis Clínics, Barcelona, Spain

4. Servei de Pneumologia, Hospital Universitari Germans Trias i Pujol, Institut d'Investigació Germans Trias i Pujol, Universitat Autònoma de Barcelona, Badalona, Spain

5. Instituto Aragonés de Ciencias de la Salud, Zaragoza, Spain

6. Hospital Universitario Miguel Servet, Zaragoza, Spain

Abstract

ABSTRACT The aim of this study was to evaluate a pyrosequencing method for the detection of Mycobacterium tuberculosis isolates resistant to rifampin and isoniazid using both clinical strains and clinical samples, comparing the results with those of the Bactec 460TB and GenoType MTBDR plus assays. In comparison to Bactec 460TB as the gold standard, the sensitivity of pyrosequencing for detecting isoniazid and rifampin resistance was 76.9% and 97.2%, respectively, for clinical strains, and the specificity was 97.2 and 97.9%, respectively. For clinical specimens, the sensitivity and specificity for both drugs were 85.7% and 100%, respectively. The overall concordance between pyrosequencing and the GenoType MTBDR plus assay for clinical strains was 99.1%, and for clinical samples, it was 98.2%. Pyrosequencing is a valuable tool for rifampin and isoniazid resistance detection.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01239-11

Reference27 articles.

1. Molecular characterisation of Mycobacterium tuberculosis isolates in the First National Survey of Anti-tuberculosis Drug Resistance from Venezuela;Aristimuno L.;BMC Microbiol.,2006

2. Single-nucleotide polymorphism-based differentiation and drug resistance detection in Mycobacterium tuberculosis from isolates or directly from sputum;Arnold C.;Clin. Microbiol. Infect.,2005

3. Identification of acid-fast bacilli using pyrosequencing analysis;Bao J. R.;Diagn. Microbiol. Infect. Dis.,2010

4. Rapid Molecular Detection of Tuberculosis and Rifampin Resistance

5. Pyrosequencing for Rapid Detection of Mycobacterium tuberculosis Resistance to Rifampin, Isoniazid, and Fluoroquinolones

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