Adhesion of K88ab to guinea pig erythrocytes: effect on membrane enzyme activities

Author:

Caloca M J1,Soler J1,Suárez S1

Affiliation:

1. Departmento de Bioquimica y Biologia Molecular, Universidad de Leon, Spain.

Abstract

Nontoxigenic Escherichia coli strains bearing K88 fimbriae have been associated with diarrhea in piglets. We have used guinea pig erythrocytes as a model of the host cell to study the cellular alterations after adherence of purified K88ab fimbriae. Although Mg2+-dependent ATPase was inhibited (up to 61%), Na/K ATPase was not. Metabolic enzymes were not significantly affected.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Reference22 articles.

1. Beutler E. 1984. Red cell metabolism. A manual of biochemical methods p. 8-80. Grune & Stratton Inc. London.

2. .Caloca M. J. S. Suarez and J. Soler. Submitted for publication.

3. Identification of two porcine brush border glycoproteins that bind the K88ac adhesin of Escherichia coli and correlation of these glycoproteins with the adhesive phenotype;Erickson A. K.;Infect. Immun.,1992

4. Determination of serum proteins by means of biuret reaction;Gornall A. G.;J. Biol. Chem.,1949

5. Inhibition of adhesive activity of K88 fibrillae by peptides derived from the K88 adhesin;Jacobs A. A. C.;J. Bacteriol.,1987

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