Comparison of Two Serologically Distinct Ribonucleic Acid Bacteriophages I. Properties of the Viral Particles

Author:

Overby L. R.12,Barlow G. H.12,Doi R. H.12,Jacob Monique12,Spiegelman S.12

Affiliation:

1. Department of Microbiology, University of Illinois, Urbana, Illinois

2. Biochemistry Research Department, Abbott Laboratories, North Chicago, Illinois

Abstract

Overby , L. R. (University of Illinois, Urbana), G. H. Barlow , R. H. Doi, Monique Jacob, and S. Spiegelman . Comparison of two serologically distinct ribonucleic acid bacteriophages. I. Properties of the viral particle. J. Bacteriol. 91: 442–448. 1966.—Two ribonucleic acid (RNA) coliphages, MS-2 and Qβ, have been characterized physically and serologically. MS-2 has an S 20, w value of 79, a molecular weight of 3.6 × 10 6 , a density of 1.422, and p H 3.9 as its isoelectric point. Qβ has an S 20, w of 84, a molecular weight of 4.2 × 10 6 , a density of 1.439, and an isoelectric point at p H 5.3. One host ( Escherichia coli A-19) permits a distinction between the two on the basis of a marked difference in plaque size. They are distinct immunochemically, no serological cross-reaction being detectable.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

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