Highly Sensitive PCR Assay for Routine Diagnosis of African Swine Fever Virus in Clinical Samples-Reference-Cited by-同舟云学术

Highly Sensitive PCR Assay for Routine Diagnosis of African Swine Fever Virus in Clinical Samples

Published:2003-09 Issue:9 Volume:41 Page:4431-4434
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Agüero M.¹,Fernández J.¹,Romero L.¹,Sánchez Mascaraque C.¹,Arias M.¹,Sánchez-Vizcaíno J. M.²

Affiliation:

1. Centro de Investigación en Sanidad Animal (CISA-INIA), Valdeolmos, 28130 Madrid

2. Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain

Abstract

ABSTRACT This work provides a novel, highly sensitive, hot start PCR method for rapid and specific detection of African swine fever virus (ASFV) that can be used as a routine diagnostic test for ASFV in surveillance, control, and eradication programs. A confirmatory test of the specificity of this method based on restriction endonuclease analysis was also developed.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.41.9.4431-4434.2003

Reference19 articles.

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2. Arias M. and J. M. Sánchez-Vizcaíno. 2002. African swine fever (ASF) p. 119-124. In A. Morilla K.-J. Yoon and J. Zimmerman (ed.) Trends in emerging viral infections of swine. Iowa State University Press Ames.

3. Arias M. and J. M. Sánchez-Vizcaíno. 2002. African swine fever eradication: the Spanish model p. 133-139. In A. Morilla K.-J. Yoon and J. Zimmerman (ed.) Trends in emerging viral infections of swine. Iowa State University Press Ames.

4. Arias, M., J. A. Martínez Escribano, A. Rueda, and J. M. Sánchez-Vizcaíno. 1986. La peste porcina Africana. Med. Vet.3:333-359.

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