Bacterial DNA Extraction Using Individual Enzymes and Phenol/Chloroform Separation-Reference-Cited by-同舟云学术

Bacterial DNA Extraction Using Individual Enzymes and Phenol/Chloroform Separation

Published:2017-09 Issue:2 Volume:18 Page:
ISSN:1935-7877
Container-title:Journal of Microbiology & Biology Education
language:en
Short-container-title:J Microbiol Biol Educ.

Author:

Wright Mitchell Henry¹,Adelskov Joseph²,Greene Anthony Carlson²

Affiliation:

1. Division of Environmental and Biomolecular Systems, Institute of Environmental Health, Oregon Health & Science University, Portland, OR 97239-3098

2. School of Natural Sciences, Griffith University, Nathan Campus, Queensland, Australia

Abstract

Marmur (4) developed one of the first detailed comprehensive methods for purifying bacterial DNA. This procedure is now outdated, and can be difficult to follow for those with limited experience in molecular biology. Here, we provide a modernized, simplified protocol for extracting bacterial DNA and discuss how this can be incorporated into microbiology laboratory courses for biology majors.

Publisher

American Society for Microbiology

Subject

General Agricultural and Biological Sciences,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,Education

Link

https://journals.asm.org/doi/pdf/10.1128/jmbe.v18i2.1348

Reference8 articles.

1. GC-MS headspace analysis of Terminalia ferdinandiana fruit and leaf extracts which inhibit Bacillus anthracis growth

2. Production of Manganese Oxide Nanoparticles by Shewanella Species

3. Impacts of Gut Bacteria on Human Health and Diseases

4. A procedure for the isolation of deoxyribonucleic acid from micro-organisms

5. A molecular phylogenetic framework for Bacillus subtilis using genome sequences and its application to Bacillus subtilis subspecies stecoris strain D7XPN1, an isolate from a commercial food-waste degrading bioreactor

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